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Construct size (kb): 9.399999618530273

DNA: genomic

Insert lengths(kb): 6.699999809265137

Tissue: neutrophil

Gene product: early growth response 1(krox-24, zif-268) [EGR1]

Restriction digests of the clone give the following sizes (kb): HindIII--6.8, 2.7; SstI--2.7, 2.2, 1.9, 1.25, 1.1, 0.2; BglII--9.4; PvuII--2.3, 2.1, 1.65, 1.5, 0.78 (doublet); EcoRI--9.4.

The insert contains the following restriction fragments (approximate kb from the 5' end): SstI--0.1, 1.14, 2.3, 4.4; BglII--2.8; ApaI--2.0, 2.5, 3.14, 5.3.

Contains the complete gene (2 exons and 1 intron) and 5' and 3' flanking sequences. The 500 bp ApaI fragment from the promoter region is sufficient to confer responsiveness to tetradecanoyl phorbol acetate in transient transfection assays.