亚洲国产成人精品久久久国产成人一区二区三区综合区精品久久久中文字幕一区,国产a一级无码毛片一区二区三区,久久久无码,国产成人无码精品久免费,精品欧美国产一区二区三区不卡,国产成人一区二区三区影院,国产精品久久久久久,欧美日韩精品一区二区三区,欧美日韩在线精品一区二区三区激情福利综合,在线观看亚洲精品福利片,亚洲欧美日韩久久精品,亚洲欧美日韩国产成人精品,亚洲国产欧美日韩精品一区二区三区,欧美日韩国产成人高清视频

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > Vaccinia virus ts mutant
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
Vaccinia virus ts mutant
Vaccinia virus ts mutant
規(guī)格:
貨期:
編號(hào):B243164
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Vaccinia virus ts mutant
商品貨號(hào) B243164
Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus ts mutant
Strain IHD-W Dts47 (Dales isolate 6828)
Applications
The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U12.
Positive for Mycoplasma No
Biosafety Level 2 CDC BMBL recommends vaccination.
Isolation
derived from existing strain
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Comments
Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was assigned a Dales EM category of P, characterized by immature normal particles and mature particles with cores that are predominantly lucent at the center. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U12.
Effect on Host
Yes, Cytopathic effect: Rounding and sloughing of infected cells
CPE: enlargement, plaques with rounding and eventual sloughing
Growth Medium Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host

Recommended Host: BSC-40 (ATCC? CRL-2761
Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 31.0°C
Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent [not 100% confluent] for inoculation.
Cryopreservation
Storage temperature: -70°C
Name of Depositor S Dales, RC Condit, S Dales
Special Collection ATCC
Source
derived from existing strain
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746

This mutant strain was derived by Samuel Dales from the IHD-W strain of Vaccinia (ATCC VR-1441) which in turn was derived from the IHD-J strain.

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

Vaccination recommended for laboratory staff manipulating vaccinia, monkeypox, or cowpox viruses [CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4].

or colder

Non-permissive incubation temperature is 39.5C. This mutant was assigned a Dales EM category of P. It is assigned the Condit map location U12. This subtype is distinguishable from the parent IHD-J strain by its ability to elicit polykaryocytosis in all mammalian and avian cells tested and by its inability to induce an active hemagglutinin at the plasma membrane.

or colder

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
连江县| 集安市| 平安县| 仲巴县| 曲沃县| 永济市| 资兴市| 白玉县| 信丰县| 林口县| 镇远县| 宜兴市| 会昌县| 曲松县| 宣恩县| 尚志市| 澄迈县| 上犹县| 通辽市| 尚义县| 苏尼特右旗| 桂林市| 安塞县| 广州市| 吴堡县| 彰化县| 闽清县| 宜丰县| 潮安县| 清丰县| 舒城县| 辽阳县| 大厂| 万宁市| 疏附县| 鹤岗市| 乌兰县| 阆中市| 皋兰县| 沁阳市| 教育|