Comments |
Restriction digests of the clone give the following sizes (kb): NotI/SalI--4.1, 2.0; EcoRV--5.1, 1.0; NotI--6.1. Insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.82; ClaI--0.53; EcoRV--0.48; NcoI--0.49, 1.75; PstI--0.93, 1.30; PvuII--0.91, 0.92, 1.61; SacI--1.72. |