Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--11.0, 2.7; HindIII--11.0, 2.7; BamHI--11.0, 1.75, 1.3; PstI--6.6, 3.0, 2.2, 1.3, 0.56, 0.48; SalI--11.0, 3.15; EcoRI/HindIII--9.4, 2.8, consistent with deposited DNA. Codominant segregation of the PvuII RFLP was observed in 44 three-generation families. Enzyme(s) not detecting polymorphism: TaqI, BamHI and HindIII. |