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Size (kb): 1.9140000343322750

Vector: RR2 (plasmid)

Construction: pUC19

Marker(s):ampR

Construct size (kb): 1.914000034332275

Features: marker(s): ampR
replicon: pMB1
MCS: BssHII...NotI

vector useful for site-directed mutagenesis

Restriction digests of the clone give the following sizes (kb): BglII--1.9; BamHI--1.9; XhoI--1.9.

Insert contains the following restriction sites (approximate kb from nt 1): AvaI--0.05, 0.09; BamHI--0.05; BglII--0.04; BssHII--0.01; DraI--0.92, 0.94, 1.63; FspI--1.28; KpnI--0.11; NotI--0.17; SmaI--0.09; SspI--1.86; XbaI--0.10; XhoI--0.05.

Cloning vector containing a reduced number of restriction sites, useful for site-directed mutagenesis of inserts.

Constructed from pUC19 by elimination of many of the restriction endonuclease sites and replacement of nt 2555-640 with a smaller polylinker.

Differs from RR1 (ATCC 87076) and GenBank S70522 by the presence of a unique FspI site (T1280G) and a C1100T transition.

Temperature: 37.0°C