亚洲国产成人精品久久久国产成人一区二区三区综合区精品久久久中文字幕一区,国产a一级无码毛片一区二区三区,久久久无码,国产成人无码精品久免费,精品欧美国产一区二区三区不卡,国产成人一区二区三区影院,国产精品久久久久久,欧美日韩精品一区二区三区,欧美日韩在线精品一区二区三区激情福利综合,在线观看亚洲精品福利片,亚洲欧美日韩久久精品,亚洲欧美日韩国产成人精品,亚洲国产欧美日韩精品一区二区三区,欧美日韩国产成人高清视频

Size (kb): 2.7000000476837160

Vector: pTC191 (plasmid)

Construction: pUC19

Marker(s):ampR

Construct size (kb): 2.700000047683716

Features: insert detection: lacZ'
marker(s): ampR
replicon: pMB1
MCS: BamHI...HindIII

vector containing primer sites useful for sequencing

vector permitting construction of fusion proteins

vector permitting visual detection of recombinants

Restriction digests of the clone give the following sizes (kb): BglI--1.6, 1.15; NcoI--2.7; PvuII--2.3, 0.40.

The plasmid contains the following restriction sites (approximate kb from nt 1): BglI--0.24, 1.85; NcoI--0.45; PvuI--0.28, 2.10; PvuII--0.31, 0.67.

Expression vector designed for converting restriction sites at the end of inserts. Also permits insert detection by lacZalpha complementation and sequencing from M13 universal and reverse primers.

Constructed from pUC19 by modification of the multiple cloning site to include some unique cloning sites surrounded by some repeated sites.

Pairs of identical restriction sites are in the same reading frame to maintain lacZ' insert detection regardless of the enzyme used.

Temperature: 37.0°C