亚洲国产成人精品久久久国产成人一区二区三区综合区精品久久久中文字幕一区,国产a一级无码毛片一区二区三区,久久久无码,国产成人无码精品久免费,精品欧美国产一区二区三区不卡,国产成人一区二区三区影院,国产精品久久久久久,欧美日韩精品一区二区三区,欧美日韩在线精品一区二区三区激情福利综合,在线观看亚洲精品福利片,亚洲欧美日韩久久精品,亚洲欧美日韩国产成人精品,亚洲国产欧美日韩精品一区二区三区,欧美日韩国产成人高清视频

Construct size (kb): 8.600000381469727

DNA: cDNA

Insert lengths(kb): 4.400000095367432

Gene product: leukotoxin [lktA]

The insert includes the following sites, numbered beginning at -469 for the EcoRV site: PvuI--158, HincII--227, BglII--346, PvuII--1252, PstI--1650, EcoRV--2683, XbaI--3920.

Restriction digests of the clone give the following sizes (kb): SalI--9.4; EcoRV/SalI--4.4, 3.4, 1.3; EcoRV--5.8, 3.2.

The insert includes 2 open reading frames; one for lktC encoding a protein of 19.8 kDa and one for lktA encoding a protein of 101.9 kDa. HB101 transformed with the plasmid shows the 100 kDa protein on western blots, but not the 20 kDa protein.