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pRS403
pRS403
規(guī)格:
貨期:
編號:B239726
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 pRS403
商品貨號 B239726
Designations pRS403
Depositors P Hieter
Biosafety Level 1
Vector Information
Size (kb): 4.456
Vector: pRS403 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pBluescriptII KS+
Marker(s):ampR,HIS3
Construct size (kb): 4.456
Features: insert detection: lacZ'
marker(s): HIS3
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
Applications
YI-type (integrating) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): PvuII--4.0, 0.45; BglI--1.7, 1.7, 1.05; PstI--3.5, 0.95.
A YI-type shuttle vector with HIS3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

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