This auxilliary vector is pUC18 with the following polylinker :
AscI -AgeI-EcoRI-SalI-PstI-NcoI-HindIII-NotI-I-AscI
Restriction digests of the vector gave the following results (in kb) : EcoRI ? 2.7 ; BglI ? 1.5, 1.2. ? ATCC staff
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Goderis IJ, et al. A set of modular plant transformation vectors allowing flexible insertion of up to six expression units. Plant Mol. Biol. 50: 17-27, 2002. PubMed: 12139006
Related Products
component of:ATCC MBA-82
Shipped
freeze-dried
Shipping Information
Shipped: Freeze dried Escherichia coli ?DH5 alpha containing the plasmid.