產(chǎn)品名稱 |
Human rhinovirus 14 |
商品貨號(hào) |
B162851 |
Classification |
Picornaviridae, Enterovirus, Rhinovirus B |
Deposited As |
Rhinovirus type 14 |
Agent |
Human rhinovirus 14 |
Strain |
1059 |
Applications |
Virucide testing Respiratory research |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 1 mL per vial |
Storage Conditions |
-70°C or colder |
Images |
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Effect on Host |
CPE, rounding and sloughing |
Recommended Host |
H1-HeLa (ATCC® CRL-1958) |
Growth Conditions |
Temperature: 33°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-85% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere while rocking continuously. End adsorption by adding virus growth medium.
Incubation: 1-5 days while rocking continuously |
Name of Depositor |
RM Conant, VV Hamparian, The Ohio State University |
Special Collection |
ATCC |
Source |
Throat swab from young adult with upper respiratory illness, South Carolina, 1959 |
References |
Johnson KM, Rosen L. Characteristics of five newly recognized Enteroviruses recovered from the human oropharynx. Am. J. Epidemiol. 77(1): 15-25, 1963.
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