Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--22.6 (doublet); EcoRI/HindIII--22.6, 10.5, 5.4, 4.0, 1.65, 1.50, 1.25, 0.43. Verified to contain the correct insert sequence using the following oligonucleotides (5'-3'): GATCTGGTGAAACACCAGCGG and GCTCTCTATAGGCTTCTCCCCACTAT. The predicted product size is (bp): 135. The observed product size is (bp): 140. |