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Size (kb): 3.7000000476837160

Vector: pBJ-247 (phagemid)

Promoters: Promoter for in vitro transcription T7 (phi10)

Construction: pBluescript II SK+

Marker(s):ampR

Construct size (kb): 3.700000047683716

Features: marker(s): ampR
promoter for expression: GAL10...GAL1
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: BamHI...SacI
MCS: KpnI...EcoRI

contains easily purifiable cassette(s) for construction

shuttle vector

vector permitting RNA synthesis in vitro

vector permitting production of single-stranded DNA

Restriction digests of the clone give the following sizes (kb): BamHI--3.7; EcoRI--3.7; SacI--3.7.

The GAL10 RNA start site is disrupted, thus providing only one direction of transcription. Unless a start site for transcription is provided, little or no message should be made from inserts cloned into the GAL10 side of the promoter cassette.

No pBS polylinker sites are included in the GAL1/GAL10 promoter cassette.

Plasmid containing a BamHI-GAL1/GAL10-EcoRI cassette of 685 bp useful for making other constructions.

The order of the major features in this plasmid is: f1 ori - lacZ' - T7 promoter - Kpn1/MCS/BamHI - GST-GAL1 promoter - GAL10 promoter - EcoRI/MCS/SacI - T3 promoter - lacI - pMB1 ori - ampR.

Temperature: 37.0°C